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Various factors affecting Au antigen sensitization of glutaraldehyde-treated erythrocytes by tannic acid method were explored extensively.The main results were as follows.An increased ratio of morphologically abnormal cells in fixed cells was obtained with increasing concentrations of glutaraldehyde with which fresh cells were treated. When Au antigen was coated to the cell preparations containing high ratio of abnormal cells, the sensitized cells tended to be agglutinated non-specifically and form indistinct settling patterns. The capacities for sensitization of fixed cells did not vary appreciably with varying times of exposure to glutaraldehyde or varying concentrations of the reagent. But fixed cells was shown to have lower capacities for sensitization with increasing times for storage at 4°C.The cells treated with increasing tannic acid concentrations or increasing antigen concentrations yielded higher titers against standard antisera, but the cells treated with high concentrations of tannic acid as well as Au antigen tended to be agglutinated non-specifically and form indistinct settling patterns. Cells incubated 5min. or longer, gave essentially similar titers with standard antisera, but the uptake of Au antigen by cells was increased gradually untill 20min. The results of sensitization with Au antigen were appreciably varied with different lots of Au antigen preparations as well as different lots of cells.

A STUDY OF VARIOUS FACTORS AFFECTING AU ANTIGEN SENSITIZATION OF GLUTARALDEHYDE-FIXED ERYTHROCYTES BY TANNIC ACID METHOD