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INTENDED USE The Reagent is intended for use in performing the activated partial thromboplastin time (APTT) test, and for APTT based factor assays using ellagic acid activator. PRINCIPLE The APTT is useful as a screening tool, and as a quantitative test for the intrinsic coagulation factors. It is a simple and versatile test which is sensitive to deficiencies of all plasma clotting factors except Factor VII. However, it is mainly used to detect deficiencies in Factor VIII, IX, XI, XII and Prekallikrein. The APTT is also commonly used to monitor heparin theraphy since APTT prolongation is directly proportional to increasing amounts of heparin. The APTT test is performed by adding reagent containing a plasma activator and phospholipid to the test specimen. This mixture is incubated for 3 minutes at 37°C for optimum activation. Calcium chloride is added and clot formation is timed. SAMPLE Plasma in trisodium citrate 3.2 % (0.109 M). Avoid hemolysis and contamination by tissue fluids. Centrifuge blood for 15 minutes at 1500 x g. Test within 2 hours if samples are held at 22-24°C. KIT COMPONENTS

Warning! Warning! Prolonged APTT!! ^|^hellip;may end in grave situation