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The effects of alpha lipoic acid and vitamin C supplementation on TGF a and TGF b2 secretion in the testicular tissue of streptozotocin-induced diabetic rats were examined by immunohistochemical methods. Rats were divided into 5 groups; group 1 (control), 2 (diabetic control), 3 (diabetes + alpha lipoic acid), 4 (diabetes + vitamin C), and 5 (diabetes + alpha lipoic acid + vitamin C). Hematoxylin and eosin staining was performed in order to examine testicular tissue histopathologically. The streptavidin-biotin-peroxidase complex method was used for histological examination and determination of TGF a and TGF b2 localization in the testicular tissue. Regarding fasting blood glucose levels, the lowest blood glucose level was detected in group 3. Fewer pathological histopathological findings were observed in groups treated with alpha lipoic acid, vitamin C, and alpha lipoic acid + vitamin C compared to the diabetes group. TGF a and TGF b2 secretions increased in groups 3, 4, and 5 compared to group 2. In conclusion, exogenous administration of alpha lipoic acid, vitamin C, and alpha lipoic acid + vitamin C increased TGF a and TGF b2 secretion in streptozotocin-induced diabetic rats.

türk veterinerlik ve hayvancılık dergisi/turkish journal of veterinary and animal sciences

Effects of alpha lipoic acid and vitamin C supplementation on transforminggrowth factor alpha (TGF α) and transforming growth factor beta 2 (TGF β2)secretion in testicular tissue of diabetic rats*