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We report primer pairs for 30 new gene regions in the nuclear genomes of   Lepidoptera   that can be amplified using a standard PCR protocol. The new primers were tested across diverse   Lepidoptera  , including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of   Lepidoptera  . We feel that a PCR-based approach still has its place in molecular systematic studies of   Lepidoptera  , particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.

PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera