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Extensive sampling for aquatic insects was conducted in the orders   Ephemeroptera   (mayflies),   Plecoptera   (stoneflies), and   Trichoptera   (caddisflies) (EPT) of Isle Royale National Park ( ISRO ), Michigan, United States of America, during summer 2013. The island was ice covered until 8,000 to 10,000 years ago and is isolated by 22–70 km distance from the mainland. Two hypotheses were examined: that  ISRO  EPT richness would be much reduced from the mainland, and that the species colonizing  ISRO  would be of smaller size than mainland, adults presumably using updrafts to bridge the distance from mainland sources. Data sets were developed for known mainland EPT species and size for those species. The first hypothesis was confirmed with the mainland species pool consisting of 417 EPT, while  ISRO  is known to support 73 species. Richness of EPT is directly related to the number of specimens examined. Small streams supported five EPT species, while 15–25 species were found in larger streams. Lakeshores had intermediate diversity. The second hypothesis was substantiated for stoneflies, but not for mayflies or caddisflies. Stoneflies apparently are poorer fliers than either of the other two orders.

Ephemeroptera , Plecoptera , and Trichoptera on Isle Royale National Park, USA, compared to mainland species pool and size distribution