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Three new species of    Trichosetodes   , namely    T.   carmelae    sp. nov. ,    T.   katiengensis    sp. nov.  and    T.   ratanakiriensis    sp. nov.  are described and illustrated by male specimens. The male genitalia of    T.   carmelae    sp. nov.  can be distinguished from the other 16 species of the genus found in Southeast Asia by the shape of the phallicata. The phallicata of    T.   carmelae    sp. nov.  bears a tuft of long hairs in the middle of the dorsal edge.    Trichosetodes   katiengensis    sp. nov.  can be distinguished from the other species in Southeast Asia by the shape of the phallicata which is divided into dorsal and ventral branches in lateral view, and    T.   ratanakiriensis    sp. nov.  by the characters of the left inferior appendage and the shape of segment IX. The posterior end of the left inferior appendage of    T.   ratanakiriensis    sp. nov.  is not forked and the ventral and lateral views of the posteroventral lobes of segment IX are rounded. Illustrations of male genitalia of    Trichosetodes   kampongspeuensis    Malicky &amp; Kong, 2020 are provided for comparison. The molecular diversity of new    Trichosetodes    species was analyzed using the mitochondrial large subunit ribosomal rRNA gene region (16S rRNA). In terms of their genetic divergence,    T.   ratanakiriensis    sp. nov.  and    T.   kampongspeuensis    exhibited remarkable proximity, with only a 1.4% distance. On the contrary,    T.   carmelae    sp. nov.  displayed genetic disparity exceeding 6.3% when compared to both    T.   ratanakiriensis    sp. nov.  and    T.   kampongspeuensis   .

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﻿New species of the genus    Trichosetodes    Ulmer, 1915 (  Trichoptera  ,   Leptoceridae  ) from Ratanakiri province, Cambodia, based on morphological and molecular data

﻿New species of the genus Trichosetodes Ulmer, 1915 ( Trichoptera , Leptoceridae ) from Ratanakiri province, Cambodia, based on morphological and molecular data

New species of the genus Trichosetodes Ulmer, 1915 ( Trichoptera , Leptoceridae ) from Ratanakiri province, Cambodia, based on morphological and molecular data