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In the Croatian fauna, horseflies (  Tabanidae  ) are represented by 78 species belonging to two subfamilies, five tribes, and 10 genera. Identification of these species is based on morphological characteristics. In this study, 43 species of horseflies were analyzed. The highest number of species (19) belongs to the genus    Tabanus   , followed by the genera    Hybomitra    with seven species,    Haematopota    with six species,    Chrysops    with four species,    Atylotus    and    Philipomyia    with two species each, and the genera    Silvius   ,    Dasyrhamphis   , and    Heptatoma    with one species each. The standard DNA barcoding region of the mitochondrial cytochrome c oxidase gene, subunit I ( COI ), was sequenced and compared to the Barcode of Life Database ( BOLD ). Our analyses confirmed our morphological identifications and added 16 new Barcode Index Numbers ( BINs ) for   Tabanidae   to  BOLD . Potential problems in the systematics and taxonomy of this family are highlighted.

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﻿DNA barcoding of the horsefly fauna (  Diptera  ,   Tabanidae  ) of Croatia with notes on the morphology and taxonomy of selected species from   Chrysopsinae   and   Tabaninae

﻿DNA barcoding of the horsefly fauna ( Diptera , Tabanidae ) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae

DNA barcoding of the horsefly fauna ( Diptera , Tabanidae ) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae