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Neottieae   comprise about 150–200 species and are distributed mainly in temperate and subtropical zones of the northern hemisphere. Mycoheterotrophy is common in   Neottieae  . Based on three DNA markers and a broad sampling of   Neottieae  , these results indicate that   Neottieae   is strongly supported as monophyletic and    Palmorchis    is sister to the remaining genera of   Neottieae  .    Holopogon    and    Neottia    s.s. are deeply nested within    Listera   . The habit of leafless mycotrophy has independently evolved at least three times in   Neottieae  , one in    Cephalanthera   , another in    Neottia    s.l. and the third in the clade formed by    Limodorum    and    Aphyllorchis   .

Molecular systematics and the evolution of mycoheterotrophy of tribe Neottieae ( Orchidaceae , Epidendroideae )