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We studied the allopatric complex    Pulsatilla   patens    (L.) Mill. s.lat. (  Ranunculaceae  ) in north-eastern European Russia and the Urals. In this region, there are two kinds of    P.   patens    with different perianth colours in monochrome and polychrome populations. To clarify their taxonomic boundaries, we used the sequences of chloroplast DNA ( rbc L and  mat K) and nuclear DNA (ITS2), in addition to morphological characteristics. The combination of three markers ( rbc L+ mat K+ITS2) was found to be the most effective for phylogenetic resolution. The samples of two morphologically-different taxa    P.   uralensis    and    P.   patents    s.str. were shown to form a single clade on the phylogenetic tree. Based on the molecular phylogenetic analysis, we were not able to unequivocally prove the independent existence of    P.   uralensis   .

phytokeys

Phylogenetic relationships and status of taxa of    Pulsatilla   uralensis    and    P.   patens    s.str. (  Ranunculaceae  ) in north-eastern European Russia