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Clinopodium   vulgare    L. (  Lamiaceae  ) was used in the traditional Bulgarian medicine for treatment of wounds, diabetes and gastric ulcers. The aim of the present study was to evaluate the antioxidant capacity of the extract ( CVE ) and fractions from    C.   vulgare    ( CV ) using  DPPH ,  ABTS  and  FRAP  methods. Enzyme inhibitory activity against acetylcholinesterase,  α -glucosidase and  α -amylase was also investigated. Rosmarinic acid was used as a positive control. The fraction CV3 demonstrated the highest radical scavenging activity with IC 50  values of 0.02 mg/ml ( DPPH ) and 0.0002 mg/ml ( ABTS ), as well as the strongest ferric reducing potential ( FRAP ) of 0.89 mM  TE /mg dw. The crude aqueous-methanol extract of    C.   vulgare    also showed high activity with IC 50  values of 0.05 mg/ml ( DPPH ), 0.04 mg/ml ( ABTS ) and 0.89 mM  TE /mg dw ( FRAP ). Moreover, CV3 demonstrated moderate  α -glucosidase and  α -amylase inhibitory potential.

Antioxidant and enzyme-inhibiting activity of lyophilized extract from Clinopodium vulgare L. ( Lamiaceae )