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Lignicolous freshwater fungi represent one of the largest groups of   Ascomycota  . This taxonomically highly diverse group plays an important role in nutrient and carbon cycling, biological diversity and ecosystem functioning. The diversity of lignicolous freshwater fungi along a north-south latitudinal gradient is currently being studied in Asia. In this paper, we introduce two novel freshwater taxa viz.    Tingoldiago   hydei    sp. nov.  and    T.   clavata    sp. nov.  which were collected from freshwater substrates in Eastern Thailand. Morphological comparison based on the size of ascomata, asci and ascospores, as well as multi-gene phylogenetic analyses based on  LSU ,  SSU ,  ITS  and  TEF1-α  DNA sequences, supports their placement in    Tingoldiago    (  Lentitheciaceae  ). Descriptions and illustrations of these two new species are provided.

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Lignicolous freshwater   ascomycota   from Thailand: Phylogenetic and morphological characterisation of two new freshwater fungi:    Tingoldiago   hydei    sp. nov. and    T.   clavata    sp. nov. from Eastern Thailand