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The genus    Tuber    is a lineage of diverse ectomycorrhizal, hypogeous, sequestrate ascomycete fungi that are native to temperate forests in the Northern Hemisphere. Recently, many new species of    Tuber    have been described in North America and Asia, based on morphological characteristics and molecular data. Here we describe and illustrate a new species,    Tuber   incognitum   , based upon phylogenetic analysis and morphological description. We also present a new record for    Tuber   anniae    in México. These two    Tuber    species are distributed in the Transmexican Volcanic Belt in the states of México, Michoacán, Guanajuato, Querétaro and Tlaxcala at altitudes between 2,000 and 3,200 meters. These species are associated with    Pinus    (   T.   anniae   ) and    Quercus    forests (   T.   incognitum   ).

Description and distribution of Tuber incognitum sp. nov. and Tuber anniae in the Transmexican Volcanic Belt