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: Based on traditional medicine, many countries use various plant products (fruits, leaves and other plant parts) as food supplements or in the form of tea. The use of these plant sources has been established through the years of use and the proven benefits of their ingredients to improve human health.Aim : In the present study, we have focused on the effect of    Lycium   barbarum    fruit extract and methylxanthines isolated from  Pu-erh  (MXP) and  Bancha  (MXB) tea leaves on Herpes simplex virus type 1 ( HSV-1 ), poliovirus 1 ( PV1 ) and coxsackievirus B1 ( CVB1 ) virus in vitro.Materials and methods : We used in vitro antiviral and virus attachment assays to determine the effects of the three extracts we studied.Results : None of the extracts showed significant inhibition of replication of the three treated viruses but a remarkable inhibitory effect on extracellular virions of  HSV-1  was exhibited 30 minutes after exposure, especially by the    Lycium   barbarum    extract. The inhibitory effect of the three extracts on the level of adsorption of the  HSV-1  to sensitive cells ( MDBK ) was also significant, with the most pronounced effect of the MXP. The protective effect of the extracts against herpes infection on healthy cells was also determined, the MXP showing the most notable effect.Conclusions : The three studied extracts can be used effectively in the treatment of herpes infections, as well as in infections with other enveloped viruses.

In vitro antiviral activities of fruit extract from Lycium barbarum and methylxanthines extracted from Pu-erh and Bancha tea leaves