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Introduction :    Pimpinella   anisum    is a medicinal plant with antimicrobial, antifungal, and anti-oxidative properties. Limited studies have assessed the antibacterial properties of    Pimpinella   anisum    on oral and dental pathogens.Aim : This  in vitro  study aimed to assess the antibacterial effect of    Pimpinella   anisum    essential oil on    Enterococcus   faecalis   ,    Lactobacillus   casei   ,    Actinomyces   naeslundii   , and    Aggregatibacter   actinomycetemcomitans   .Materials and methods : After obtaining the essential oil of    Pimpinella   anisum   , its antimicrobial activity was evaluated using the agar disc diffusion test. The minimum inhibitory concentration and minimum bactericidal concentration of the essential oil were also determined; 0.2% chlorhexidine was used as the positive control.Results : The mean diameter of growth inhibition zone was 39 mm for    Enterococcus   faecalis   , 40 mm for    Lactobacillus   casei   , 42 mm for    Actinomyces   naeslundii   , and 18.5 mm for    Aggregatibacter   actinomycetemcomitans   . The mean diameter of the growth inhibition zones for    Enterococcus   faecalis   ,    Lactobacillus   casei   , and    Actinomyces   naeslundii    was significantly greater than that of    Aggregatibacter   actinomycetemcomitans    ( p =0.001). Also, the mean diameter of the growth inhibition zone of    Actinomyces   naeslundii    was significantly larger than that of    Enterococcus   faecalis    ( p =0.05). The minimum inhibitory concentration and minimum bactericidal concentration of the essential oil for    Enterococcus   faecalis    were 4.88% and 4.88%, respectively. These values were 9.76% and 9.76% for    Lactobacillus   casei   , 9.76% and 4.88% for    Actinomyces   naeslundii   , and 9.76% and 9.76% for    Aggregatibacter   actinomycetemcomitans   , respectively.Conclusions :    Pimpinella   anisum    essential oil was effective against all four microorganisms evaluated in this study. Since the lowest minimum inhibitory concentration and minimum bactericidal concentration were recorded for    Enterococcus   faecalis   , this essential oil has maximum effects on    Enterococcus   faecalis   . Future clinical studies are required to assess the antimicrobial efficacy of    Pimpinella   anisum    essential oil in clinical samples.

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In vitro antibacterial effect of    Pimpinella   anisum    essential oil on    Enterococcus   faecalis   ,    Lactobacillus   casei   ,    Actinomyces   naeslundii   , and    Aggregatibacter   actinomycetemcomitans