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Chromosome studies along with heterochromatin distribution pattern analysis have been carried out in two domesticated species of    Vigna    Savi, 1824 which grow in contrasting geo-climatic conditions of India:    Vigna   umbellata    Thunberg, 1969, a legume well acclimatized to subtropical hilly regions of North-east India and    V.   aconitifolia    Jacquin, 1969, a species of arid and semi-arid regions in desert plains of Western India. Karyo-morphological studies in both species reveal 2 n  = 22 chromosomes without any evidence of numerical variation and the overall karyotype symmetry in chromosome morphology suggest that the diversification at intraspecific level in genus    Vigna    has occurred through structural alteration of chromosomes, rather than numerical changes. Heterochromatin distribution as revealed by fluorochrome binding pattern using CMA 3  and DAPI, confirms the occurrence of relatively more GC content in    V.   aconitifolia    as compared to    V.   umbellata   . However, AT content was found to be comparatively higher in    V.   umbellata    which perhaps play a role in species interrelationships.

comparative cytogenetics

Heterochromatin distribution and comparative karyo-morphological studies in    Vigna   umbellata    Thunberg, 1969 and    V.   aconitifolia    Jacquin, 1969 (  Fabaceae  ) accessions