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To increase the number of cytogenetic characters used in    Ololygon   tripui    systematics, we applied some cytogenetic techniques such as Giemsa, C- and NOR-banding, and    fluorescence  in situ  hybridization    (FISH)  with 18S rDNA and repetitive microsatellite DNA probes to the study of four populations from Minas Gerais State (southeastern Brazil). All populations showed 2n = 24 and FN = 48, and chromosomal formula 8m + 10sm + 6st.    Nucleolar organizing regions   (NORs)  were located on chromosome pair 6 in all populations, although in the Tripuí locality additional markings were observed on one homologue of chromosome pair 3. These patterns were partially congruent with results obtained using the 18S rDNA  FISH  probe. The microsatellites repetitive DNA (GA) 15  and (CAT) 10  probes accumulated predominantly in the terminal region of all chromosomes. Chromosome morphology and Ag-NOR were conserved among populations, a conserved pattern in    Ololygon    Fitzinger, 1843. Repetitive DNA  FISH  probes patterns were similar among populations, but they revealed species-specific differences when compared with other species of the genus    Ololygon   , suggesting that molecular cytogenetics are potentially more informative in karyologically conservative taxa.

comparative cytogenetics

Karyological study of    Ololygon   tripui    (Lourenço, Nascimento and Pires, 2009), (  Anura  ,   Hylidae  ) with comments on chromosomal traits among populations