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The aim of the present study was to compare cellular susceptibility and oxidative stress response of    S.   cerevisiae    logarithmic ( log ), quiescent (Q), and non-quiescent ( NQ ) cell populations to menadione – a well-known inducer of oxidative stress. Three main approaches were used: microbiological – cell survival, molecular – constant field gel electrophoresis for detection of DNA double-strand breaks ( DSB ), and biochemical – measurement of reactive oxygen species ( ROS ) levels, oxidized proteins, lipid peroxidation, glutathione, superoxide dismutase ( SOD ) and catalase on    S.   cerevisiae    haploid strain BY4741. The doses causing 20% (LD 20 ) and 50% (LD 50 ) lethality were calculated. The effect of menadione as a well-known oxidative stress inducer is compared in the  log , Q, and  NQ  cells. Survival data reveal that Q cells are the most susceptible to menadione with LD 50  corresponding to 9 µM menadione. On the other hand, dose-dependent  DSB  induction is found only in Q cells confirming the results shown above. No effect on  DSBs  levels is observed in  log  and  NQ  cells. Further, the oxidative stress response of the cell populations is clarified. Results show significantly higher levels of  SOD  and  ROS  in Q cells than in  log  cells after the treatment with 100 µM menadione. On the other side, higher induction of oxidized proteins, malondialdehyde, and glutathione is observed after menadione treatment of  log  cells. Our study provides evidence that    Saccharomyces   cerevisiae    quiescent cells are the most susceptible to the menadione action. It might be suggested that the DNA damaging and genotoxic action of menadione in    Saccharomyces   cerevisiae    quiescent cells could be related to  ROS  production.

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﻿Cellular susceptibility and oxidative stress response to menadione of logarithmic, quiescent, and nonquiescent    Saccharomyces   cerevisiae    cell populations

﻿Cellular susceptibility and oxidative stress response to menadione of logarithmic, quiescent, and nonquiescent Saccharomyces cerevisiae cell populations

Cellular susceptibility and oxidative stress response to menadione of logarithmic, quiescent, and nonquiescent Saccharomyces cerevisiae cell populations