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Here we present a general collecting and preparation protocol for DNA barcoding of   Lepidoptera   as part of large-scale rapid biodiversity assessment projects, and a comparison with alternative preserving and vouchering methods. About 98% of the sequenced specimens processed using the present collecting and preparation protocol yielded sequences with more than 500 base pairs. The study is based on the first outcomes of the Indonesian Biodiversity Discovery and Information System (IndoBioSys). IndoBioSys is a German-Indonesian research project that is conducted by the  Museum für Naturkunde  in Berlin and the  Zoologische Staatssammlung  München, in close cooperation with the Research Center for Biology – Indonesian Institute of Sciences (RCB-LIPI, Bogor).

biodiversity data journal

A streamlined collecting and preparation protocol for DNA barcoding of   Lepidoptera   as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)