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BackgroundMembers of the family   Mycetophilidae   (  Diptera  ) have life cycles that are typically associated with fungus. Their biology is relatively well known in the Palaearctic, though other regions are poorly known, and there are no associations recorded between mycetophilid immatures and fungi in the Neotropical region. Here we report the first association between a mycetophilid—   Neoempheria   puncticoxaEdwards—and fungi in this region. Immatures ofN.   puncticoxawere collected on fungi and some were reared in the laboratory until adult emergence. The immature stages and adult ofN.   puncticoxaare described and re-described respectively, and high resolution images and illustrations of the habitus, wings, thorax, male and female terminalia, immatures, and  in situ   specimens are given.New informationWe report the first association between   Mycetophilidae   and fungi in the Neotropical region.

biodiversity data journal

First record of    Neoempheria    Osten Sacken (  Diptera  ,   Mycetophilidae  ) biology in the Neotropical region, with associations between its larvae and fungi