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Indo-China floristic region is among the 34 richest floristic regions of the world, and its plant diversity is still under investigation. Here we report a new record of an aquatic plant,    Potamogeton   distinctus   , from Myanmar, a part of the region, that is detected by means of DNA barcoding method. The molecular method further identified the other specimens as hybrids of    Potamogeton   : one is   P.   ×  malainoides   (   P.   distinctus    ×    P.   wrightii   ), and the other is    P.   distinctus    ×    P.   nodosus   . The first of these was thus far genetically confirmed in China, but the parental combination of the hybrid in Myanmar was reciprocal to those reported from China. The second hybrid was also recorded from China, but the maternal lineage was revealed for the first time, in this case it was    P.   distinctus   . The present study showed that 1) nrITS is useful to distinguish closely related    Potamogeton    species as well as hybrids among them and 2)  atpB - rbcL  has higher utility than other frequently used plastid DNA markers. We thus propose nrITS and  atpB - rbcL  as DNA barcoding markers for future    Potamogeton    studies.

biodiversity data journal

DNA barcoding reveals a new record of    Potamogeton   distinctus    (  Potamogetonaceae  ) and its natural hybrids,    P.   distinctus    ×    P.   nodosus    and    P.   distinctus    ×    P.   wrightii    (   P.   ×  malainoides   ) from Myanmar