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BackgroundTibetan Plateau is one of the most typical areas of biodiversity in the world because of its unique environmental and regional units, which breed unique biological communities and concentrate on many unique and rare wild animals and plants. Research on   Chironomidae   in the Tibetan Plateau is relatively weak. At present, the identification of   Chironomidae   species mainly depends on male adults, while identification of larvae and pupae is relatively difficult and there is less research on them.New informationDuring the investigations of insect diversity in the Tibetan Plateau, larval and pupal stages of    Orthocladius   nitidoscutellatus    Lundström, 1915 and    Psectrocladius   nevalis    Akhrorov, 1977 were described and illustrated. Matching and identification of larval and pupal stages were based on DNA barcodes. Neighbour-joining trees were reconstructed, based on known    Orthocladius    and    Psectrocladius    COI DNA barcodes, respectively.

biodiversity data journal

New descriptions of the larval and pupal stages of    Orthocladius   nitidoscutellatus    and    Psectrocladius   nevalis    from Xizang, China (  Diptera  ,   Chironomidae  )