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Chaeturichthys   stigmatias    Richardson, 1844, a fish species of the family   Gobiidae  , is an offshore warm-temperate fish species and a dominant component of estuarine ecosystems. In this study, restriction site-associated DNA sequencing was adopted to analyze the traits of candidate microsatellite markers for    C.   stigmatias   , and 30 polymorphic loci were developed. A total of 5631 microsatellites with primer fragments were detected, among which trinucleotide repeats (57.56% of the total simple sequence repeats) were the most abundant, followed by di- (23.30%), tetra- (11.79%), penta- (4.14%), and hexa- (3.21%) nucleotide repeats type. The numbers of alleles per locus ranged from 6 to 14 with the mean value of 10.4. The mean value of observed heterozygosity and the expected heterozygosity were 0.349 and 0.870, respectively. The microsatellite locus with the lowest polymorphic information content ( PIC ) was 0.749, which indicated that all sites were highly polymorphic ( PIC  &gt; 0.50). This is the first microsatellite development and characterization of this species to be reported.

acta ichthyologica et piscatoria

﻿Development and characterization of microsatellite markers for    Chaeturichthys   stigmatias    (  Actinopterygii  :   Gobiiformes  :   Gobiidae  ) based on restriction site-associated DNA sequencing (RAD-seq)

﻿Development and characterization of microsatellite markers for Chaeturichthys stigmatias ( Actinopterygii : Gobiiformes : Gobiidae ) based on restriction site-associated DNA sequencing (RAD-seq)

Development and characterization of microsatellite markers for Chaeturichthys stigmatias ( Actinopterygii : Gobiiformes : Gobiidae ) based on restriction site-associated DNA sequencing (RAD-seq)