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Breast cancer is the most common type of cancer among women worldwide. Researchusing breast cancer cell lines derived from primary tumors may provide valuableadditional knowledge regarding this type of cancer. Therefore, the aim of thisstudy was to investigate the phenotypic profiles of MACL-1 and MGSO-3, the onlyBrazilian breast cancer cell lines available for comparative studies. We evaluatedthe presence of hormone receptors, proliferation, differentiation and stem cellmarkers, using immunohistochemical staining of the primary tumor, cultured cellsand xenografts implanted in immunodeficient mice. We also investigated the abilityof the cell lines to form colonies and copy number alterations by array comparativegenomic hybridization. Histopathological analysis showed that the invasive primarytumor from which the MACL-1 cell line was derived, was a luminal A subtype carcinoma,while the ductal carcinoma in situ (DCIS) that gave rise to the MGSO-3 cell linewas a HER2 subtype tumor, both showing different proliferation levels. The celllines and the tumor xenografts in mice preserved their high proliferative potential,but did not maintain the expression of the other markers assessed. This shiftin expression may be due to the selection of an 'establishment' phenotype in vitro.Whole-genome DNA evaluation showed a large amount of copy number alterations (CNAs)in the two cell lines. These findings render MACL-1 and MGSO-3 the first characterizedBrazilian breast cancer cell lines to be potentially used for comparative research.

Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors