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Gene transfer using different viral vectors has demonstrated different antitumoreffects in suicide gene therapy. In the present study, in order to optimize theefficacy of replication-defective adenoviral and lentiviral vectors for gene therapy,RT-PCR was used to evaluate the expression of Drosophila melanogaster deoxyribonucleosidekinase (Dm-dNK) in the Bcap37 human breast cancer cell line, dThd was used todetermine the activity of Dm-dNK, cell cytotoxicity was evaluated by MTT assayand cell proliferation was assessed using a hemocytometer. Moreover, apoptosisinduction was evaluated by the Annexin V-FITC-labeled FACS method. Furthermore,BALB/C nude mice bearing tumors were treated with Dm-dNK mediated with the pyrimidinenucleoside analog, brivudine [BVDU, (E)-5-(2-bromovinyl)-2'-deoxyuridine]. Ourresults indicated that the gene expression of Dm-dNK transfected by adenoviraland lentiviral vectors may be detected and that its long-term activity may beretained. Both vectors containing the Dm-dNK gene revealed high cytotoxicity andsensitized cell apoptosis from the BVDU prodrug. In tumor models, lentivirus-mediatedgene therapy significantly inhibited the growth of tumors compared with adenovirus-mediatedgene therapy. Although adenovirus- and lentivirus-transduced Dm-dNK reveal strongtreatment efficacy in vitro, the latter has great potential due to the long-termexpression of the therapeutic gene in vivo.

oncology reports

Cytotoxic effects of adenovirus- and lentivirus-mediated expression of Drosophila melanogaster deoxyribonucleoside kinase on Bcap37 breast cancer cells