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The Bmi1 gene has been reported to play important roles in cancer initiationand progression. The aim of this study was to investigate the effects of RNA interference(RNAi)-mediated silencing of Bmi1 gene expression on the proliferation and invasivenessof hepatocellular carcinoma (HCC) cells and on the efficacy of chemotherapy inHCC patients. The Bmi1 gene was silenced by Bmi1-siRNA (small interfering RNA)in the human HCC cell lines HepG2 and Bel-7402, and the gene expression levelswere assayed by real-time quantitative reverse transcription-polymerase chainreaction (qRT-PCR) and western blotting. The proliferation and migration of Bmi1-silencedtumor cells and their sensitivity to 5-FU treatment were determined by Cell CountingKit-8 (CCK-8), transwell assays and 4',6-diamidino-2-phenylindole (DAPI) stainingand flow cytometry, respectively. Bmi1-siRNA inhibited the Bmi1 expression atboth the mRNA and protein levels in HCC cells. Proliferation and migration ofHCC cells treated with Bmi1-siRNA was significantly lower compared to that ofthe control cells. Moreover, Bmi1 gene silencing increased the percentage of apoptoticcells treated by 5-FU and decreased the IC50 values of 5-FU to a greater extent.Downregulation of the Bmi1 gene by RNAi can inhibit the proliferation and invasivesnessof HCC cells and increase their sensitivity to 5-FU treatment.

oncology reports

Bmi1 gene silencing inhibits the proliferation and invasiveness of human hepatocellular carcinoma cells and increases their sensitivity to 5-fluorouracil