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Colorectal cancer (CRC) is one of the major causes of cancer-related mortalityworldwide. Recent studies revealed that there is a relationship between CRC occurrenceand microRNA (miRNA) function. Stem cells are a type of cells that have the abilityto self-renew and to proliferate extensively while maintaining the undifferentiatedstate. Cancer stem cells (CSCs) are closely linked to tumor recurrence and metastasis.To this end, we evaluated the miRNA expression differences between colon CSCsand non-stem cells using the SW1116 cell line, to determine the relationship betweentumor stem cells and tumor biological behavior. We isolated populations of colonCSCs with the CD133+/CD44+ and CD133-/CD44- surface phenotype from a human SW1116colon adenocarcinoma cell line using flow cytometry. The expression of miRNA andmRNA of both sets of cells was examined with miRNA and mRNA arrays. Bioinformaticmethods were used to analyze microarray results. We completed gene ontology analysis,pathway analysis, miRNA target gene prediction with databases. We identified acolon stem cell miRNA expression profile comprising 31 upregulated and 31 downregulatedmiRNAs, such as miR29a, miR29b, miR449b and miR4524. Some of these differentiallyexpressed miRNAs may be involved in the regulation of stem cell differentiation.Gene ontology and pathway analyses showed that the differences are closely relatedto the function of the cell cycle, cell differentiation, signaling pathway, cytoskeletalproteins and cell-matrix adhesion in colon cancer stem cells. We found that miRNAsplay an important role in regulating the expression of colon CSC characteristics.By regulating the expression of CSC signaling pathways, cytoskeleton and membraneproteins, miRNAs give tumor stem cells the macrobiological behavior of recurrenceand metastasis. This study provides a new perspective on CRC metastasis and recurrence.

miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line