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Toll-like receptor 3 (TLR3) is a pattern-recognizing receptor that is involvedin immune signaling and plays a crucial role in survival by being able to recognizevarious viral components including double-stranded RNA (dsRNA). TLR3 expressionand function in cancer cells are not well understood. In this study, we investigatedwhether TLR3 agonist dsRNA (BM-06) can inhibit proliferation and invasion, andpromote apoptosis in HepG2.2.15 cells. HepG2.2.15 cells secreting hepatitis Bvirus (HBV) were treated with BM-06 and poly(I:C). Western blot analysis and PCRwere employed to determine pharmacodynamic changes in biomarkers relevant to TLR3signaling. Cell proliferation, invasion and apoptosis were analyzed by CCK-8 assay,transwell assay and flow cytometry. The expression of HBsAg, and HBcAg was observedby immunohistochemistry. Compared with untreated cells, pharmacological NF-κBactivity of the TLR3 pathway by BM-06 (1.734-fold) or poly(I:C) (1.377-fold) wasinduced. By western blot analysis, we found that dsRNA induced TLR3-activatedHepG2.2.15 cells which expressed NF-κB levels predominantly in the cytoplasmicfraction but fewer signals in the nucleus. BM-06 inhibited the proliferation,invasion and secretion of HBV, and induced apoptosis in HepG2.2.15 cells. In addition,the antitumor effects of BM-06 were superior to poly(I:C). Pharmacological activationof the TLR3 pathway by BM-06 can inhibit HepG2.2.15 cell growth.

TLR3 dsRNA agonist inhibits growth and invasion of HepG2.2.15 HCC cells