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It has been recently reported that side population (SP) cells in nasopharyngealcarcinoma (NPC) cell lines display characteristics of cancer stem-like cells.However, the biological behavior and the significance of these cells for NPC progressionremain unclear. In this study, we isolated SP cells from the NPC cell line CNE-2by flow cytometry and investigated their biological characteristics. We discoveredthat SP cells had stronger colony forming abilities compared to the non-side population(NSP) cells, and observed that some SP cells looked more like the shape of mesenchymalcells when cultured in the common polyHEMA-coated flask. When checked by quantitativereal-time PCR, the SP cells expressed higher levels of stemness-related genesOct4, Sox2 and Nanog, and mesenchymal cell-related genes N-cadherin, vimentinand Snail, while they expressed lower levels of the epithelial cell-related gene,E-cadherin. Western blot and immunofluorescence staining methods further verifiedthat SP cells expressed higher vimentin and expressed lower E-cadherin levels.Finally, Transwell invasion assay results indicated that the SP cells had higherinvasive potential compared to NSP cells. Collectively, our data reveal that SPcells in the CNE-2 cell line not only possess the properties of cancer stem cells,but also have more mesenchymal cell characteristics which are associated withepithelial mesenchymal transition (EMT) and cancer cell invasion and metastasis.These findings are helpful for developing novel targets for effective clinicaltreatment of NPC.

oncology reports

Cancer stem-like side population cells in the human nasopharyngeal carcinoma cell line cne-2 possess epithelial mesenchymal transition properties in association with metastasis