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Omphalia lapidescens is an important medicinal fungus as well as traditionalChinese medicine used for disease treatment. It is mainly used as a vermifugefor anthelmintic therapy, but it has not been hitherto reported to possess antitumoractivity. In this study, a purified bioactive protein in O. lapidescens (pPeOp)was obtained using polyvinylpyrrolidone (PVP) followed by gel filtration chromatography.To evaluate the in vitro antitumor activity of pPeOp in human gastric tumor cells(MC-4 and SGC-7901) and normal cells (MC-1), MTT assay and FCM assay were usedand the morphological changes, cell viability, cell death rate and cell apoptosisrate of MC-4, SGC-7901 and MC-1 cells were estimated. The results showed thatpPeOp could significantly reduce the cell viability of MC-4 and SGC-7901 cellsin a concentration-dependent manner, with IC50 values of 236.05 and 156.28 µg/ml,respectively. The morphological observation also indicated a similar result. InFCM assays, a significant increase of cell death rate and cell apoptosis rateof the tumor cells were observed, indicating probable necrosis-inducing effectsand/or apoptosis-inducing effects of pPeOp. Importantly, there was no significanteffect of pPeOp on MC-1 cells in each assay, showing that pPeOp has no adverseeffects on the normal cells. In conclusion, pPeOp is a newly discovered bioactiveprotein in O. lapidescens and this is the first report on antitumor activity ofsuch a fungal protein. This may provide a meaningful basis for developing a newprotein drug for treatment against cancer, especially gastric cancer.

A PVP-extract fungal protein of Omphalia lapideacens and its antitumor activity on human gastric tumors and normal cells