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The present study investigated the expression and clinical significance of micro-ribonucleic acid-23a (miR-23a) and metastasis suppressor 1 (MTSS1) in diffuse large B-cell lymphoma (DLBCL). A total of 70 cases of tumor tissues of patients with DLBCL and 30 cases of reactive lymphoid hyperplasia tissues were collected. OCI-LY10 cell was transfected with miR-23a antisense oligonucleotide (miR-23a ASO). The expression of miR-23a and MTSS1 in tumor tissues of patients with DLBCL and reactive lymphoid hyperplasia tissues were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry. Spearmans test was used for correlation analysis was also performed for their expression. The relationship of the expressions of miR-23a and MTSS1 with the pathological parameters of patients with DLBCL was further analyzed. The DLBCL OCI-LY10 cells were cultured in vitro , and gene silencing downregulated the expression of miR-23a in OCI-LY10 cells. The expression of miR-23a was studied via RT-qPCR, and the effect of downregulation of miR-23a on MTSS1 protein expression was determined by western blot analysis. Moreover, the effects of miR-23a on the proliferation, metastasis and invasion capacities of OCI-LY10 cells were observed by both methyl thiazolyl tetrazolium (MTT) assay and Transwell chamber assay. The results of RT-qPCR showed that the mRNA expression of miR-23a in DLBCL tissues was significantly higher than that of reactive hyperplasia tissues. Immunohistochemical results revealed that the positive expression rate of MTSS1 in DLBCL tissues (30.00%) was significantly lower in comparison to reactive hyperplasia tissues (90.00%). Correlation analysis revealed that the miR-23a expression had a significant negative correlation with MTSS1 expression (r=-0.538, p=0.01). The expression of miR-23a and MTSS1 were correlated with the Ann Arbor staging, extranodal invasion and International Prognostic Index (IPI) scores of patients (p&lt;0.05). However, they had no significant correlation with the sex and age of patients (p&gt;0.05). After the downregulation of miR-23a expression, the MTSS1 protein expression in OCI-LY10 cells showed a significant increase. However, the proliferation, metastasis and invasion capacities of OCI-LY10 cells were obviously decreased. In conclusion, miR-23a promoted the proliferation, invasion and metastasis of DLBCL OCI-LY10 cells through the targeted inhibition of MTSS1. The high expression of miR-23a and the low expression of MTSS1 protein could be used as reference indexes for the prognosis of DLBCL.

Expression and clinical significance of miR‑23a and MTSS1 in diffuse large B‑cell lymphoma