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Hypertrophic scars are characterized by the abnormal proliferation of fibroblastsand an overproduction of collagen. The Sp1 transcription factor is involved inthe stimulation of collagen synthesis. A decoy oligonucleotide (ODN) targetingSp1 was designed and transfected into hypertrophic scar fibroblasts (HSFs) cellsusing cationic liposomes. The transfection efficiency was determined by flow cytometryand was observed to be 85±7% (n=5). Specific binding of the Sp1 decoy ODN wasmonitored with an electrophoretic mobility shift assay (EMSA). Following transfectionwith the decoy ODN to Sp1, cell viability and cell proliferation, which were examinedby the cell counting kit WST‑8, were decreased by 80% compared with untreatedcells. Transforming growth factor‑β (TGF‑β) mRNA and collagen mRNA expressionwere also reduced by 48% in the transfection decoy ODN group. The cell viabilityof HSFs after 48 h of transfection with 25, 50, 100 and 150 nM Sp1 decoy ODN was0.9331±0.0203, 0.7479±0.0868, 0.577±0.0347 and 0.4703±0.0147, respectively. The100 nM dose of the Sp1 decoy ODN inhibited the expression of types I and III collagenby 32 and 28%, respectively (both P&lt;0.01). TGF‑β mRNA expression was also effectivelysuppressed by the 100 nM Sp1 decoy ODN (P&lt;0.01). The Sp1 decoy ODN inhibitedcell proliferation and the expression of types I and III collagen. Therefore,Sp1 decoy ODNs may be a promising tool for developing and testing novel therapeuticapplications for treating hypertrophic scars.

Suppression of cell proliferation and collagen production in cultured human hypertrophic scar fibroblasts by Sp1 decoy oligodeoxynucleotide