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Essential oils have a sedative effect on stress, and are also known to haveantibiotic and anti-carcinogenic effects. These compounds have long been usedas natural microbial agents, and have recently been added to a number of pharmaceutical,food and cosmetic products. Controlling the exposure to allergens and pathogensare important factors for the treatment of allergy, and potentially reducing therisk of sensitization and infection. Low humidity, at levels under 35%, may affecthuman comfort and health during the winter. Patients and other individuals requireoptimal humidification to maintain a moisturized respiratory tract necessary forprotecting against bacterial infection. We designed an analytical system to examinethe effects of aromatherapeutic essential oils on airborne bacteria. The antibacterialactivities of essential oils were assayed using agar plate air-sampling methods.A bacterial suspension was sprayed into a bio-clean room through the upper holesusing a spray gun. Free-floating airborne bacteria were collected from the bio-cleanroom (blank) in blood agar plates for 10 sec using an air sampler. Three differentconcentrations of essential oils (0.0005, 0.005 and 0.05 ppm) were then sprayedinto the bio-clean room for 5 min. Free-floating airborne bacteria were collectedevery 10 min for 10 sec each. Treatment with 0.0005 ppm essential oils inhibitedthe growth of colonies; this effect appeared to persist after 60 min. Decreasedbacterial colony growth was more apparent in the presence of 0.005 ppm and 0.05 ppmessential oils than 0.0005 ppm. These effects were observed after 60 min comparedto the control (distilled water). These results indicate that essential oils areable to inhibit the growth of airborne bacteria.

Analysis of the effects of essential oils on airborne bacteria in a customized bio-clean room