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The aryl hydrocarbon receptor (AHR) is a basic helix-loop-helix (bHLH) transcriptionfactor that is activated by environmental contaminants including polychlorinatedbiphenyls (PCBs). The AHR affects a variety of processes that are involved incell growth and differentiation. In this study, we constructed a P19 embryoniccarcinoma cell line with AHR gene silencing using the vector-based approach ofshort hairpin (sh)RNA interference that allows cells to differentiate into cardiacmyocytes when treated with dimethyl sulfoxide (DMSO). The expression levels ofthe cardiac development-specific GATA4 and Nkx2.5 genes were measured using real-timequantitative polymerase chain reaction (qPCR). Our data showed that the expressionlevels of the GATA4 and Nkx2.5 genes were increased in the AHR-silenced P19 cellscompared with the control groups. Four critical genes (ARNT, CYP1A1, GSK3β andβ-catenin) expressed in the AHR and in the Wnt signaling pathway were also measuredby qPCR. We found that the expression levels of ARNT, CYP1A1 and β-catenin weresuppressed, whereas GSK3β expression was elevated in the AHR-silenced P19 cells.Therefore, it is possible that the silencing of AHR promotes the differentiationof P19 cells through the AHR and Wnt signal transduction pathway.

ShRNA-mediated gene silencing of AHR promotes the differentiation of P19 mouse embryonic carcinoma cells into cardiomyocytes