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Histamine (HA), a mediator of inflammation, type I allergic responses andneurotransmission, is synthesized from L-histidine, the reaction of which is catalyzedby histidine decarboxylase (HDC). HDC has been reported to be induced by variousstimuli, not only in mast cells and basophils, but also in T lymphocytes and macrophages.Although its mRNA has been shown to be increased in Jurkat cells when treatedwith phorbol 12-myristate 13-acetate (TPA), little is known concerning the inducedproduction of HA by HDC. The present study quantified the trace amounts of intracellularHA using ultra-high liquid chromatography in combination with the 6-aminoquinolinecarbamate-derivatization technique. To test whether the cellular level of HA iselevated by the induction of HDC in Jurkat cells treated with TPA, the peak correspondingto authentic HA in the cell lysate was fractioned and its molecular weight determinedby matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flightmass spectrometry. The results of this study show that the HA level is increasedby the induction of HDC expression by TPA in Jurkat cells. Therefore, this methodis useful in elucidating the physiological significance of HA production.

Enhanced histamine production through the induction of histidine decarboxylase expression by phorbol ester in Jurkat cells