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This study was carried out to investigate the effect of fibronectin (FN)on primary cultured human fetal hepatocytes infected by hepatitis B virus (HBV)in vitro. Human fetal hepatocytes infected by HBV were cultured on culture dishescoated with FN in vitro. HBsAg and HBeAg in supernatants were detected by ELISA,HBcAg in nuclei by immunohistochemistry, HBV DNA by fluorescent quantitative PCRand covalently closed circular DNA (cccDNA) by nested PCR. The results were comparedwith cells cultured on dishes that were not coated with FN. Positivity for HBsAgand HBeAg in supernatants appeared from day 5 on the non-coated dishes, whilepositivity for HBsAg and HBeAg appeared from day 1 on the coated dishes. Positivityfor HBcAg in the nuclei on the non‑coated dishes was displayed from day 2 witha positive rate of 15%, while positivity for HBcAg on the coated dishes was displayedfrom day 1 with a positive rate of &gt;90%. HBV DNA in cells on the non-coateddishes was detected from day 4, while that on the coated dishes was detected fromday 1. The non-coated dishes were positive for cccDNA from day 8, while the coateddishes were positive from day 2. FN coating is capable of accelerating HBV infectionin primary cultured fetal hepatocytes in vitro, increasing the duration of HBVinfection and the number of infected cells.

Effect of fibronectin on HBV infection in primary human fetal hepatocytes in vitro