English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Primary effusion lymphoma (PEL) is a subtype of B-cell lymphoma caused byhuman herpes virus 8/Kaposi sarcoma-associated herpes virus (HHV-8/KSHV), whichis mostly found in patients with AIDS and has poor prognosis. Nuclear factor (NF)-κBpathway is constitutively activated in HHV-8-infected PEL cells and plays a crucialrole in tumorigenesis. Recently, it has been shown that diethyldithiocarbamate(DDTC), an active metabolite of disulfiram, has apoptotic activity in cancer cells.Here, we investigated the effect of DDTC on PEL using a PEL mouse model generatedby intraperitoneal injection of BC-3 cells, a PEL cell line. DDTC amelioratedthe symptoms of PEL in these mice, such as development of ascites, splenomegalyand increase of body weight, in comparison with PBS-treated controls. Moreover,we determined in vitro that DDTC suppressed the constitutively activated NF-κBpathway in BC-3 cells. Methylthiotetrazole assay revealed that the cell proliferationof various PEL cell lines was significantly suppressed by the treatment of DDTC.DDTC also induced the expression of cleaved caspase-3, an apoptosis marker, whereasthe addition of Q-VD-OPh, a pan-caspase inhibitor, inhibited cell apoptosis inducedby DDTC treatment. Together, our results indicated that DDTC induces apoptosisvia inhibition of the NF-κB signaling pathway in HHV-8-infected PEL cells. Thisstudy suggests the potential use of DDTC as a therapeutic approach for PEL.

Diethyldithiocarbamate induces apoptosis in HHV-8-infected primary effusion lymphoma cells via inhibition of the NF-κB pathway