English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Breast cancer is the leading cause of cancer-related deaths in women worldwide.Tanshinone IIA (Tan-IIA) is one of the pure compounds from Salviae miltiorrhizaeradix (Danshen). Tan-IIA can inhibit human breast cancer cells but the molecularmechanisms are not well understood. Our previous study showed that Tan-IIA caninhibit hep-J5 human hepatocellular carcinoma cells through the endoplasmic reticulum(ER) stress-induced apoptotic pathway. In the present study, we evaluated theeffects of Tan-IIA on BT-20 human breast cancer cells and assessed the involvementof the ER-stress-apoptotic pathway. The cytotoxicity of Tan-IIA in BT-20 cellswas measured by the MTT assay. The cell cycles were analyzed by flow cytometry.The expression of ER stress-related proteins in BT-20 cells treated with Tan-IIAwere evaluated by western blotting and immunocytochemical staining. These resultsshowed that Tan-IIA can inhibit BT-20 cells and increase the sub-G1 phase in atime- and dose-dependent manner. Tan-IIA could increase the protein expressionof caspase 12, GADD153, caspase 3, phospho-JNK, phospho-p38 and Bax, but decreasedBcl-xl and phospho-ERK expression in BT-20 cells. These findings indicate thatTan-IIA possesses therapeutic potential for human breast cancer BT-20 cells; oneof the molecular mechanisms may be through inducing ER stress and the MAPK pathwayto induce apoptosis and inhibit proliferation.

Tanshinone IIA inhibits BT-20 human breast cancer cell proliferation through increasing caspase 12, GADD153 and phospho-p38 protein expression