English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

The effect of β 2 -adrenergic receptor (AR) overexpression on interleukin (IL)-10 content secreted by cardiomyocytes of heart failure (HF) rats was investigated. A rat model of chronic HF was established by partially banding abdominal aorta and the cardiomyocytes were isolated with collagenase II. The cardiomyocytes were then transfected with adenovirus type 5-ADRβ 2 -enhanced green fluorescent protein (EGFP) for 48 h to observe the changes of β 2 -AR protein expression using western blot analysis. The IL-10 level was detected by ELISA. The experiment was divided into seven groups: Control, HF, HF+EGFP, HF+β 2 , sham, sham+EGFP and sham+β 2 groups. Compared with the sham-operated group, left ventricular diastolic dimension, and left ventricular systolic dimension were increased (P&lt;0.05), whereas ejection fraction and fractional shortening were decreased (P&lt;0.05) in the HF group. Compared with the sham group, the cardiomyocyte survival rate of the HF group was significantly reduced (P&lt;0.05). Compared with the control or sham group, the β 2 -AR protein level of the HF group showed no significant differences (P&gt;0.05). Compared with the HF and HF+EGFP groups, the expression of β 2 -AR protein of cardiomyocytes was increased in the HF+β 2 group (P&lt;0.05). Compared with the sham group, IL-10 content secreted by cardiomyocytes in the HF group was increased (P&lt;0.05). Compared with the HF and HF+EGFP groups, IL-10 content in the HF+β 2 group was increased significantly (P&lt;0.05). In conclusion, the concentration of IL-10 secreted by cardiomyocytes of HF rats was increased. The overexpression of β 2 -AR in the cardiomyocytes of HF rats was able to enhance the secretion of IL-10.

Effect of adenovirus mediated β2-AR overexpression on IL-10 level secreted by cardiomyocytes of heart failure rats