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Star-to-star light-element abundance variations, know as multiple stellarpopulations (MPs), are common in almost all Galactic globular clusters.Recently, MPs have also been detected in a number of massive clusters with agesin excess of 2 Gyr in the Large Magellanic Cloud (LMC), thus indicating thatage is likely a control parameter for the presence of MPs. However, toconclusively confirm this notion, additional studies of star clusters in theLMC's `age gap' of 3--6 Gyr are required. Here, we use {\sl Hubble SpaceTelescope} observations to study the 3 Gyr-old cluster NGC 2121. Compared withso-called `simple' stellar population models, the cluster's red-giant branchexhibits an apparent spread in a specific color index that is sensitive tointrinsic chemical spreads. The observed spread can be explained by anintrinsic spread in nitrogen abundance of $\sim$0.5--1.0 dex}. NGC 2121 has acomparable mass to its younger counterparts without MPs, indicating thatcluster mass might not be the dominant parameter controlling the presence ofMPs. The transition phase between the occurrence of clusters with or withoutMPs seems to occur at ages of 2--3 Gyr, indicating that cluster age may play adominant role in the establishment of MPs.

When does the onset of multiple stellar populations in star clusters occur? Detection of enriched stellar populations in NGC 2121