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Using the Green Bank 100 m telescope and the Nobeyama 45 m telescope, we haveobserved the rotational emission lines of the three 13C isotopic species ofHC3N in the 3 and 7 mm bands toward the low-mass star-forming region L1527 inorder to explore their anomalous 12C/13C ratios. The column densities of the13C isotopic species are derived from the intensities of the J = 5-4 linesobserved at high signal-to-noise ratios. The abundance ratios are determined tobe 1.00:1.01 +- 0.02:1.35 +- 0.03:86.4 +- 1.6 for[H13CCCN]:[HC13CCN]:[HCC13CN]:[HCCCN], where the errors represent one standarddeviation. The ratios are very similar to those reported for the starlesscloud, Taurus Molecular Cloud-1 Cyanopolyyne Peak (TMC-1 CP). These ratioscannot be explained by thermal equilibrium, but likely reflect the productionpathways of this molecule. We have shown the equality of the abundances ofH13CCCN and HC13CCN at a high-confidence level, which supports the productionpathways of HC3N via C2H2 and C2H2+. The average 12C/13C ratio for HC3N is 77+- 4, which may be only slightly higher than the elemental 12C/13C ratio.Dilution of the 13C isotope in HC3N is not as significant as that in CCH orc-C3H2. We have also simultaneously observed the DCCCN and HCCC15N lines andderived the isotope ratios: [DCCCN]/[HCCCN] = 0.0370 +- 0.0007 and[HCCCN]/[HCCC15N] = 338 +- 12.

Precise observations of the 12C/13C ratios of HC3N in the low-mass star-forming region L1527