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Problem statement: No specific antiviral therapy is currently available despite an emergence and resurgence of Japanese encephalitis in South-East Asian Countries. There are only few recent studies, which were aimed to treat Japanese encephalitis with newer drugs. There is thus a real need for study on antiviral agents that can reduce the toll of death and neurological sequelae resulting from infection with this virus. Approach: Optimum dilution of the JE virus was determined which could produce significant number of pocks on Chorioallantoic Membrane (CAM). Then ultradiluted belladonna preparations were used to see their inhibitory action on JE virus infection in CAM. Results: Ultradiluted belladonna showed significantly decreased pock count in CAM in comparison to JE virus control. Conclusion: Ultradiluted belladonna could inhibit JE virus infection in CAM, which may be mediated through glycosidase inhibitory role of calystegines present in belladonna

Decreased Intensity of Japanese Encephalitis Virus Infection in Chick Chorioallantoic Membrane Under Influence of Ultradiluted Belladonna Extract