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Problem statement: The formation of a urinary stone, known as &#13; nephrolithiasis, urolithiasis, renal calculi or kidney stone is a serious, debilitating &#13; problem in all societies throughout the world. Struvite or Ammonium Magnesium &#13; Phosphate Hexahydrate (AMPH) is one of the components of urinary stone (calculi). &#13; Struvite stones are commonly found in women. Struvites form in humans as a result &#13; of urinary tract infection with ureolithic urea splitting micro organisms. These &#13; stones can grow rapidly forming "staghorn-calculi", which is more painful &#13; urological disorder. Therefore, it is of prime importance to study the growth &#13; and inhibition of Struvite crystals. Approach: This in vitro &#13; study had been carried out in the presence of herbal extract of Boerhaavia diffusa &#13; Linn. by using single diffusion gel growth technique. Sodium metasilicate solution &#13; of specific gravity 1.05 and an aqueous solution of ammonium dihydrogen phosphate &#13; of 0.5 M concentration were mixed so that the pH value 7.0 could be set. After &#13; the gelation, equal amount of supernatant solution of 1.0 M magnesium acetate &#13; prepared with 0.5 and 1% concentrations of the herbal extract of B. diffusa Linn. &#13; were gently poured on the set gels in the respective test tubes in the aseptic &#13; medium. Results: The growth of crystals without and with herbal &#13; extracts was monitored at regular time intervals. As the concentration of B. diffusa &#13; Linn. increased, the inhibition of crystals also increased in the gel media as &#13; well as the dissolution of crystals at the gel-liquid interface increases. The &#13; de-fragmentation of some grown crystals was also noticed. Conclusion: &#13; The herbal extract of B. diffusa Linn. inhibited the growth of struvite &#13; crystals in vitro. This study incorporated multidisciplinary interests and may &#13; be used for formulating the strategy for prevention or dissolution of urinary &#13; stones

Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Boerhaavia diffusa Linn