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Problem statement: The present investigation carry out a pilot study of a novel method to&#13; identify the salivary volatiles in different phases of menstrual cycle for the assessment of ovulation&#13; detection using gas chromatography and mass spectrophotometer. Approach: The profiles from&#13; follicular phase (6-12 days); ovulatory phase (13-14 days) and luteal phase (15-26 days) of menstrual&#13; cycle samples were compared to establish any qualitative and quantitative differences that might have&#13; potential value in human olfactory communication. Dichloromethane was used as the solvent for&#13; extraction of the compounds. Results: Fifteen compounds were identified. They include organic&#13; compounds like, acid, aldehyde, amine and alcohol. The most important constituent was 2-nonenal,&#13; which usually comprised 75% or more of the total volatiles observed in ovulatory phase. The&#13; concentration of many constituents varied widely. This appeared to be periodically in three cycles for&#13; five of the constituents, with a period of a few weeks and with pronounced maxima at the peak of&#13; ovulatory period of which only two were common to all the chromatograms. The chemical profile of&#13; ovulatory phase saliva was distinguished by the presence of two specific compounds, viz. 2-&#13; Nonenal, Acetic acid and Acetaldehyde that were not found in the other reproductive phases of&#13; saliva sample in women. Apparently these compounds are 2-nonenal, dodecanol, acetic acid and&#13; acetaldehyde. One or more of these compounds may have pheromonal activity in human body odor.&#13; Conclusion: Differentiation of the volatile patterns among reproductive phases in women may help to&#13; find the diagnostic marker for ovulation detection

2-Nonenal-Ovulatory Specific Volatiles in Human Saliva throughout Menstrual Cycle by Gas Chromatography and Mass Spectrometry Analysis