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Problem statement: Boar sperm cells are sensitive to the freezing process, which&#13; compromises viability of frozen-thawed sperm. In a constant search for minimizing or suppressing&#13; sperm cell damage caused by the temperature and osmolarity changes during the freezing process,&#13; crioprotective and antioxidant substances have been added to the freezing media, such as coconut&#13; water, in order to increase the viability of frozen-thawed swine semen. The addition of any substance&#13; to the freezing diluent, directly affects osmolarity of the media, which can have positive or negative&#13; effects over the sperm cell. Approach: There are no published studies currently that indicate the effect&#13; of adding coconut water over the osmolarity of freezing media and their effect over viability of sperm&#13; cells, therefore, the objective of the present study was to evaluate the effect of coconut water based&#13; diluents osmolarity over the Motility (Mot), Acrosome Integrity (AI), Membrane Integrity (MI) and&#13; Mitochondrial Activity (MA) of thawed boar sperm cells. The treatments used were control T1 (LEY&#13; with bidistilled water + LEYGO) with an osmolarity range of 296-368 mOsmol Kg-1, T2 (LEY and&#13; deionized coconut water + LEYGO) between 381 and 480 mOsmol Kg-1 and T3 (LEY and in natura&#13; coconut water + LEYGO) between 519 and 1041 mOsmol Kg-1. The Westendorf modified method&#13; was the freezing method used. The obtained data were statistically analyzed by GLM, using the SAS&#13; software (SAS, 2000). Results: A significant difference was observed on T2 compared to T1 regarding&#13; Mot 41.9 Vs 36.9% and MI 58.0 Vs 50.2. T3 had a detrimental effect on all studied variables.&#13; Conclusion: Under our study conditions, the osmolarity range of T2, due to the non ionic solutes&#13; content, contributed to improve the viability of frozen-thawed sperm cells

Osmolarity of Coconut Water (Cocos nucifera) Based Diluents and their Effect Over Viability of Frozen Boar Semen