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Problem statement: Pseudomonas spesies are one of the rihizobacterial group that have an&#13; important role in plant growth promoter and plant health. To prepare them as inoculants, they must&#13; have a range of characters as growth promoter such as Indole Acetic Acid (IAA) producers which can&#13; promote the growth of plants and solubilize phosphates. In addition, they must also have the various&#13; characters that act as biocontrol agents such as siderofor, chitinase and anti-fungal compound&#13; producers. Approach: Pseudomonas sp isolated from soybeans rhizospere and identified based on&#13; physiological reactions and 16S rRNA gene sequences. Various tests for the determination of the&#13; growth promoter were based on IAA production, phosphate solubilization and growth promoter of&#13; length of root and stems and number of lateral roots of soybean sprouts. Test of siderophore, chitinase,&#13; as well as anti anti-fungal compounds productions to inhibit the growth of Fusarium oxysporum,&#13; Rhizoctonia solani and Sclerotium rolfsii, were used as a biocontrol agent determination.&#13; Hypersensitivity test was used to screen for Pseudomonas sp classified as non-pathogenic&#13; rhizobacteria. Results: Fourteen isolates identified as a non-pathogenic Pseudomonas sp that produced&#13; IAA and Promoted enhancement of root length, shoot length, or number of lateral root. Among those&#13; 14 isolates, 8 isolates showed phosphate solubilizing activity, 12 isolates capable of producing&#13; siderophore and six isolates were observed to have chitinolytic activity. Only three isolates were able&#13; to inhibit the growth of Fusarium oxysporum in high level. While one and two isolates inhibited&#13; Sclerotium rolfsii and Rhizoctonia solani in high level, respectively. Conclusion: On the basis of&#13; excellent growth promoter and biocontrol activities, we recommended 5 isolates of Pseudomonas sp&#13; which were Crb-3, Crb-16, Crb-17, Crb-44 and Crb-94 as potential isolates of Pseudomonas sp that&#13; could be applied as inoculants of soybean plant

Screening of <i>Pseudomonas</i> sp. Isolated from Rhizosphere of Soybean Plant as Plant Growth Promoter and Biocontrol Agent