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Sucrose-glucan glucosyltransferase (Gtf) is an importantenzyme involved in the cavity formation process where insolubleglucan is synthesized. In this study, we purified Gtf from Streptcoccusmutans Ingbritt through ammonium sulfate precipitation, SephadexG-150, CM-Sephadex, and DEAE-Sephadex column chromato-graphies. A 13-fold of purification was achieved with a total yieldof 6.3%. The apparent molecular mass of the enzyme was determinedto be 66 kDa on sodium dodecyl sulfate-polyacrylamide gelelectrophoresis (SDS-PAGE). The optimal pH and temperaturewere established to be 6.0 and 40 o C, respectively. The enzymeactivity could be inhibited to 22–59% by 1 mM Hg 2+ , Cu 2+ andAl 3+ , and to 68% by 1 mM EDTA. It was also inhibited 40% by2 mM xylitol and 35–45% by 0.05% soluble chitosan, glycolchitosan, and glycol chitin. This is the first report to reveal theinhibition effect of chitin derivatives on Gtf activity, which maybe further applicable to develop gargles to overcome cavity.Keywords glycol chitin · glycol chitosan · Streptococcus mutansIngbritt · sucrose-glucan glucosyltransferase · xylitol         Streptococcus mutans. S. mutans       carigenicity (Harno , 1982; Choi, 1984).S. mutans sucrose-glucan glucosyltransferase (Gtf)     (Carson , 1969),  2 Gtf (Gtf-I, Gtf-S) fructosyltransferase (Ftf)     (Kenny Cole,1983). Gtf        ,   S. mutans      (Tanzer , 2008).S. mutans 3 Gtf . Gtf B α-1,3    , Gtf D α-1,6  , Gtf C α-1,3  α-1,6   (Aoki , 1986; Hanada Kuramitsu, 1988; 1989). 3 Gtf (gtfB, gtfC, gtfD) S. mutans GS-5  (Kuramitsu, 1993; Fujiwara , 1998). S. mutans Gtf B, C, D    (endocarditis) interleukin-6  cytokine   (modulins)  (Shun , 2005)., Gtf     . Gtf-I    8M    (Hamada , 1989; Mukasa , 1989).  Gtf-S ,    S. mutans       7  (KennyCole, 1983).    dextransucrase    Gtf

Characterization of Streptococcus mutans Ingbritt Sucrose-glucan Glucosyltransferase and the Inhibition Effect of Chitin Derivatives on its Activity