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We investigated the effects of woody plant colonization of abandoned pastures\udon soil and litter organic carbon (C) stocks and nitrogen (N) content along a\udbioclimatic transect in a semi-arid environment (Sicily, Italy). Soil samples\udwere taken in three successional stages (grazed pasture, shrubland, forest)\udwithin each of three bioclimates (supramediterranean - “supra”, mesomediterranean\ud- “meso”, thermomediterranean - “thermo”). Organic C and N in litter\udand soil (0-10 cm and 10-30 cm depth) were determined, as well as soil bulk\uddensity. Especially at 0-10 cm depth, changes in C and N contents along successional\udstages differed among bioclimates. Soil organic carbon (SOC) stock\uddecreased from pasture to shrubland and increased from shrubland to forest in\ud“supra”, increased from pasture to shrubland and then remained stable in\ud“thermo”, and was stable in “meso”. Soil C/N ratio decreased with succession\udin “supra”, showed no significant trend in “meso”, and increased with succession\udin “thermo”. Litter C stock increased with succession in “meso”, increased\udfrom pasture to shrubland and decreased from shrubland to forest in\ud“thermo”, and increased from pasture to shrubland and then remained stable\udin “supra”. Litter C/N ratio increased in “thermo” and “supra” from pasture to\udshrubland and from shrubland to forest, but did not change significantly with\udsuccession in “meso”. The different trends in SOC among bioclimates may be\udcaused by changes in the importance of litter input, litter decay rate and mineralization.\udSuccessional changes in “meso” and “supra” appeared to be most\udaffected by litter quality, while those in “thermo” appeared to be strongly influenced\udby limited litter decay due to low soil moisture and high temperature

The effects of post-pasture woody plant colonization on soil and aboveground litter carbon and nitrogen along a bioclimatic transect