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Hepatitis C is an international public health problem. It has been estimated that further than 170 million persons globally have chronic hepatitis C (CHC) infections, and about 10% to 20% of them will grow severe liver diseases such as cirrhosis and hepatocellular cancer (Perz and Alter 2006). Its causal agent, hepatitis C virus (HCV), is a small, positive‐sense, single‐stranded RNA virus(Kim and Chang 2013). HCV endemic is compounded by lacking public health resources and a lack of knowledge of Hepatitis C mostly between the at-risk population health care providers, and policymakers(Mitchell, Colvin, and Beasley 1992).

A Complete method of Detection of HCV Either Positive or Negative