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The soyabean is an important dietary component which contains abundant amount of proteins or amino acids required by human body for normal growth and maintenance. In this project, isolation of soya proteins is carried out by extraction with 0.01N sodium bicarbonate and thereafter homogenization, centrifugation and dialysis of extract against Tris buffered saline solution (pH 8.8). These proteins are characterized qualitatively by electrophoresis and quantitatively by Biuret assay method. Biuret assay method effectively quantifies the amount of protein in a given sample. Amino acids present in the isolated protein were identified and quantified using standard or marker amino acids. The stability study of isolated soya proteins is carried out in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF), prepared according to protocol specified in USP. These proteins were quantified by Biuret assay method after respective time interval in above specified fluids. The soya proteins are found to hydrolyze in SGF after 5 minutes and in SIF within one hour. Thus in-vitro stability study of proteins in simulated fluids is an effective approach and alternative to animal model for bio-stability assessment.

ISOLATION, CHARACTERIZATION, ANALYSIS AND STABILITY STUDY OF SOYA PROTEINS IN SIMULATED FLUIDS