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The aim of the present study is to evaluate anthelmintic activity of crude ethanolic extract of leaf rachis of Azadirachta indica linn. (neem) against pheretima posthuma as test worm. Four concentrations (10 mg/mL, 15 mg/mL, 25 mg/ml, 50 mg/ml) of ethanolic extract were studied in the activity, which involved the determination of the time taken for paralysis (P) and the time taken for death (D) of the worms. Albendazole (10 mg/ml) was included as standard reference and saline water as control. The results of present study indicated that crude ethanolic extract exhibited significant anthelmintic activity at concentration 50 mg/ml compared to reference Albendazole. The anthelmintic activity of ethanolic extract of Leaf Rachis of Azadirachta Indica linn therefore being demonstrated for the first time and showed the best anthelmintic activity.

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTHELMINTIC ACTIVITY OF ETHANOLIC EXTRACT OF LEAF RACHIS OF AZADIRACHTA INDICA Linn. (NEEM)